Oligonucleotide ligation assay pdf

This protocol describes the oligonucleotide ligation assay (OLA), which uses a set of three oligonucleotides, in combination with a thermostable Taq DNA ligase enzyme, to discriminate single-nucleotide polymorphism (SNP) alleles. Sixteen-plex OLA genotyping reactions are carried out, and allele-specific OLA products are detected on membrane. oligonucleotide ligation assay (OLA) A method to identify a specific oligonucleotide sequence without use of radiochemicals, electrophoresis or centrifugation. An oligonucleotide biotinylated at its 5'-end and another with a reporter group (chromophore or fluorophore) at its 3'-end are constructed to hybridize to the sequence to be detected in a template DNA strand. Combined PCR-oligonucleotide ligation assay for rapid detection of Salmonella serovars Article (PDF Available) in Journal of Clinical Microbiology 33(11) · November with Reads.

Oligonucleotide ligation assay pdf

pdf. Comparison of Oligonucleotide Ligation Assay and Consensus Sequencing for Detection of Drug-Resistant Mutants of Human Immunodeficiency Virus Type 1 in Peripheral Blood Mononuclear Cells and Plasma. Journal of Clinical Microbiology, Sarah Holte. Combined PCR-oligonucleotide ligation assay for rapid detection of Salmonella serovars Article (PDF Available) in Journal of Clinical Microbiology 33(11) · November with Reads. This protocol describes the oligonucleotide ligation assay (OLA), which uses a set of three oligonucleotides, in combination with a thermostable Taq DNA ligase enzyme, to discriminate single-nucleotide polymorphism (SNP) alleles. Sixteen-plex OLA genotyping reactions are carried out, and allele-specific OLA products are detected on membrane.tation of the oligonucleotide ligation assay (OLA). The . the principle that DNA ligase can join two adjacent Oligonucleotide primers for both amplification and. bacteria by both culture and the PCR-OLA procedure (% sensitivity), and . mer oligonucleotides used in the ligation assay was used to determine the sen-. Oligonucleotide ligation assay combined with polymerase chain reaction. (PCR- OLA) is a . The principle of PCR-OLA is described in. Figure 1. As the ®rst step .

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How to perform an MLPA reaction - by MRC-Holland, time: 10:34
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Ligase chain reaction (LCR), time: 6:13
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2 Comments

  1. Interesting theme, I will take part. I know, that together we can come to a right answer.

  2. Very similar.

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